Table 2 Rh59 (UL35) deletion reduces dissemination of 68-1 RhCMV vectors.

Three naturally RhCMV-infected RMs (T2-1, T2-2, and T2-3) were co-inoculated with 107 PFU each of 68-1/SIVrtni (left arm) and ΔRh59/SIVgag (right arm). One RM was necropsied at 14, 21, or 28 dpi, and viral genome copy numbers per 107 cell equivalents in the indicated tissues were determined using ultrasensitive nested qPCR specific for SIVrtni (68-1) or SIVgag (ΔRh59). Normalized to 1 × 107 cell equivalents.

Super infection: 68-1 versus ΔRh59
RM T2-1RM T2-2RM T2-3
Tissue type14 dpi21 dpi28 dpi
68-1/SIVrtniΔRh59/SIVgag68-1/SIVrtniΔRh59/SIVgag68-1/SIVrtniΔRh59/SIVgag
Skin injection site—
right (ΔRh59)
12<1193<136,1107
Skin injection site—
left (68–1)
541<123,793<14,480,601<1
Axillary LN3<113<12,5157
Peripheral LN<1<1<1<118<1
Liver/gallbladder<1<1<1<12<1
Heart/lung/kidney/
BAL
2,933<13<16<1
Bone marrow/spleen/
tonsil
6<1<1<18<1
Neuro/endocrine3<1<1<141
Genitourinary tract<1<13<19<1
Salivary gland2<14<13<1