Three naturally RhCMV-infected RMs (T2-1, T2-2, and T2-3) were co-inoculated with 107 PFU each of 68-1/SIVrtni (left arm) and ΔRh59/SIVgag (right arm). One RM was necropsied at 14, 21, or 28 dpi, and viral genome copy numbers per 107 cell equivalents in the indicated tissues were determined using ultrasensitive nested qPCR specific for SIVrtni (68-1) or SIVgag (ΔRh59). Normalized to 1 × 107 cell equivalents.
| Super infection: 68-1 versus ΔRh59 | ||||||
| RM T2-1 | RM T2-2 | RM T2-3 | ||||
| Tissue type | 14 dpi | 21 dpi | 28 dpi | |||
| 68-1/SIVrtni | ΔRh59/SIVgag | 68-1/SIVrtni | ΔRh59/SIVgag | 68-1/SIVrtni | ΔRh59/SIVgag | |
| Skin injection site— right (ΔRh59) | 12 | <1 | 193 | <1 | 36,110 | 7 |
| Skin injection site— left (68–1) | 541 | <1 | 23,793 | <1 | 4,480,601 | <1 |
| Axillary LN | 3 | <1 | 13 | <1 | 2,515 | 7 |
| Peripheral LN | <1 | <1 | <1 | <1 | 18 | <1 |
| Liver/gallbladder | <1 | <1 | <1 | <1 | 2 | <1 |
| Heart/lung/kidney/ BAL | 2,933 | <1 | 3 | <1 | 6 | <1 |
| Bone marrow/spleen/ tonsil | 6 | <1 | <1 | <1 | 8 | <1 |
| Neuro/endocrine | 3 | <1 | <1 | <1 | 4 | 1 |
| Genitourinary tract | <1 | <1 | 3 | <1 | 9 | <1 |
| Salivary gland | 2 | <1 | 4 | <1 | 3 | <1 |