Table 2 Comparative microscopy, qPCR/PCR, LC-MRM, and LFIA for 100 samples collected from children with subclinical parasitemia from Cameroon.

Time to readout for LFIA test line positivity and a maximum of 15 min for negative tests are included (right column).

Case codeAgeMicroscopyqPCR/PCRPSSP17
GametocyteTrophozoitePfs2518S rRNALC-MRMLFIATime to
readout
A11512++0.05765+15
A12011+++0.03222+15
A1878+++0.01779+10
A193*7+++0.02164+/−15
A22510+++0.04005+>20
A22711+++0.02615+10
A26810+++0.04036+15
A27010+++0.04381+10
A27811+++0.01951+15
A27912+++0.02216+10
A29010+++0.05321+>20
A4478+++0.03030+15
A4507+++0.03537+>20
A45210+++0.01364+15
A4608+++0.03212+>20
A4749+++0.01039++5
A4758+++0.01483+10
A49310+++0.01932++5
B00610++0.02097+10
B00710+++0.01784+>20
B0239++++0.05046+>20
B02413++0.04245++5
B0266+++0.08123+15
B0287++++0.06404++5
B02910+++0.08174+15
B0349++++0.04904+5
B04313+++0.01549+10
B04613+++0.06638+10
B0928++++0.07328+5
B0965++++0.03484+15
B1158+++0.05615+10
B1246+++0.0123115
B1267+++0.09663+15
B12810+++0.07039++5
B1457++0.02850++5
B33610++0.07140++5
B3458+++0.00521++5
B3498++0.04474+10
B35014+0.04074+15
B3608++0.0231315
B38012++0.04243++5
B38112++0.0692415
B39011++0.03683+10
B39216+++0.04583+15
C00110+++0.03759+10
C00910+++0.07004+5
C01013+++0.08945++5
C024*12+++0.06621++5
C026*10+++0.04700+15
C046*12+++0.02633+>20
C0759+++0.01026+10
C08012++0.01808++5
C084*8++0.04231+>20
C095*10++0.01362++5
C100*10++0.06038+/−10
C10611++0.0297315
C1078+0.02309+10
C112*10++0.01808+/−15
C117*12++0.10120+/−15
C1238+++0.01950++5
C3347+++0.04649+5
C3376+++0.04951+10
C3397+++0.03356+15
C3407+++0.02086+10
C3416++++0.01425+>20
C3575++0.0561615
C3588++0.02162+15
C3638+++0.04584+5
D03011+++0.03905++5
D033*13+++0.03652++5
D04010++0.03692++5
D04112++0.0147915
D0467++0.05459+5
D0499++0.02713+>20
D05312++0.04751+10
D0546+++0.0172315
D0578++0.02074+10
D0629++0.00967+>20
D15510++0.03353+15
D4469++0.02517+10
D4478++0.04891+15
D4499++0.01917+>20
D4539+++0.04835+10
D4569+++0.01120+15
D45913+++0.04252+10
D46113++0.02149++5
D46211++0.03318+15
D48210+++0.03762+10
D4879+++0.0044515
D4967++0.02504+15
D4999+0.06422+10
D5008+0.01736++5
D5168++0.03949+5
D517*9+0.02459++5
N22710++0.04180++5
N2349++0.01287+10
N24510+++0.08426+10
N2559++0.10392+15
N3118++0.04298+10
N3186+++0.04264+10

*Samples included in Fig. 3D.