Table 1 The most abundant P. falciparum proteins identified in children aged 5 to 16 years with subclinical parasitemia in Cameroon.

A total of 35 proteins were identified by LC-MS/MS with a Mascot ions score of ≥25 (table S1). Of the 35, the 19 proteins below were considered the most abundant. Score indicates the Mascot ions scores, as described previously (23). The P. falciparum female gametocyte-specific biomarker, indicated in bold, was found to have the highest Mascot ions score in our pooled sample. Mr, molecular mass in kilodaltons (kDa).

Gene IDDescriptionMr (kDa)Annotated Gene
Ontology
MS*Mascot ions score
PF3D7_0401900Acyl-CoA synthetase110.8Long-chain fatty acid–
CoA ligase activity
A28
PF3D7_0422300Alpha tubulin 249.7GTP binding, GTPase
activity, structural
molecule
A31
PF3D7_0507800Conserved Plasmodium
protein
177.4NoneA, G40
PF3D7_0509400RNA polymerase I340.7DNA binding,
DNA-directed RNA
polymerase activity
G47
PF3D7_0610400Histone H315.5DNA bindingA534
PF3D7_0705500Inositol-phosphate
phosphatase, putative
330.7NoneG31
PF3D7_0906100Developmental protein,
putative
21.9NoneA, G37
PF3D7_1102400Flavoprotein, putative78.5NoneA27
PF3D7_1134700DNA-directed RNA
polymerase 1, subunit
2, putative
175.5DNA binding,
DNA-directed RNA
polymerase activity,
ribonucleoside binding
G37
PF3D7_1215100Conserved Plasmodium
protein
113.0ATP binding, actin
binding, calmodulin
binding, motor activity
IG27
PF3D7_1216900DNA binding
chaperone, putative
111.1DNA binding, heat
shock protein binding
A36
PF3D7_1218800Conserved
Plasmodium sexual
stage protein,
putative
39.6NoneG949
PF3D7_1235700ATP synthase subunit
beta, mitochondrial
58.4Hydrogen ion
transporting ATP
synthase activity,
rotational mechanism
A116
PF3D7_1313500Conserved Plasmodium
membrane protein
209.1Hydrolase activity,
triglyceride lipase
activity
G42
PF3D7_1319200Conserved Plasmodium
protein
103.9Flavin adenine
dinucleotide binding
G33
PF3D7_1325900Conserved Plasmodium
protein
326.2ATP binding, actin
binding, calmodulin
binding, motor activity
G34
PF3D7_1337500Conserved Plasmodium
protein
393.3Calcium ion binding,
receptor activity
IG33
PF3D7_1353000Tryptophan-rich
antigen, pseudogene
96.3NoneA49
PF3D7_1411400Plastid replication-
repair enzyme
235.83′-5′ Exonuclease
activity, ATP binding,
DNA binding, DNA
helicase/polymerase
activity
G31

*MS evidence for mature gametocytes (G), immature gametocytes (IG), and asexual stages (A), based on the following published references (20, 23).