Table 2 mVIM and mCCNA1 performance in esophageal balloon samples.

VIM and CCNA1 gene methylation was assayed in DNA samples from non-endoscopic balloon sampling of the distal esophagus from unaffected controls [individuals with gastroesophageal reflux disease (GERD), erosive esophagitis, or no pathology detected during endoscopy]; cases of NDBE, further subclassified as SSBE of 1 to 3 cm or LSBE of ≥3 cm; BE with LGD; BE with HGD; EAC (including junctional cancer of the esophagus). Samples were scored as VIM-methylated for mVIM >1.0% and as CCNA1-methylated for mCCNA1 >1.0% (using ROC-defined cutpoints from Fig. 6). Samples were positive for the panel of mCCNA1 plus mVIM if either marker tested positive. Entries indicate percent sensitivity or specificity (%) and total number of individuals tested (n). We note that only four HGDs were studied, and in this small sample size, differences in the rate of mVIM and mCCNA1 detection of HGD versus detection of NBDE or EAC are not statistically significant (P > 0.088 for any between group comparisons).

mVIMmCCNA1Either mVIM or mCCNA1
%n%n%n
Specificity unaffected
controls
91.736100.03691.736
Sensitivity all cases80.05072.05088.050
Sensitivity all NDBE80.63171.03190.331
Sensitivity SSBE69.21353.81384.613
Sensitivity LSBE88.91883.31894.418
Sensitivity all
dysplastic BE
72.71172.71181.811
Sensitivity LGD83.36100.06100.06
Sensitivity HGD50.0450.0450.04
Sensitivity EAC87.5875.0887.58