Table 2. Sensitivity and specificity of RT-QuIC assay for CJD skin samples

NA, not available.

DiagnosisLower backApexArea near earOverall
sCJDVV1NA+NA+
sCJDMM1NA+NA+
sCJDVV1-2NA+NA+
sCJDMM2NA+NA+
sCJDMV2NA+NA+
sCJDMM1NA+++
sCJDMM1+/−*+++
sCJDMM1++++
sCJDMM1+++
sCJDMM1++/−++
sCJDVV2NA+++
sCJDVV2++++
sCJDVV2NA+++
sCJDVV2++++
sCJDMM2+/−++
sCJDMM2NA+++
sCJDMM1-2++++
sCJDMM1-2++
sCJDMV1-2++++
sCJDMV1-2++++
sCJDMV1++++
Sensitivity (%)928894100§
Non-CJD
Non-CJD
Non-CJDNA
Non-CJD
Non-CJD
Non-CJD
Non-CJD
Specificity (%)100100100100

*Weak positive (+/−) means one of four or two of eight wells on two or more independent experiments.

†Positive (+) RT-QuIC prion seeding activity.

‡Negative (−) RT-QuIC prion seeding activity.

§RT-QuIC analysis of skin samples from the torso or forearm of the UK vCJD and US vCJD patients, respectively, was RT-QuIC–positive and was included in the overall sensitivity and confidence interval calculations.

¶RT-QuIC analysis of skin samples from the torso or forearm of eight non-CJD patients was RT-QuIC–negative and was included in the overall specificity and confidence interval calculations.