Table 1. Classification modeling using the 261-MF biosignature list.
Classification
model
Test-Set sample
group
Number of data
files*
RF (261 MFs)LASSO (38/82 MFs)
Number correctly
predicted
% Classification
accuracy
Number correctly
predicted
% Classification
accuracy
1. Two-way modelEarly Lyme disease6058975998
STARI3834893489
2. Three-way modelEarly Lyme disease6051855185
STARI3835923592
Healthy controls4038953793

*Samples were analyzed in duplicate by LC-MS.

†A total of 38 MFs were selected by the LASSO model for two-way modeling, and 82 MFs were selected by the LASSO model for three-way modeling.