Table 2. Direct detection of the PRVABC59 Zika virus strain in cell lysates and mosquito tissue homogenates.

dpi, days post-infection; MD, mosquito diluent.

SampleZika LAMP (number of positive/total)PFU
Mock-infected cultured cells*0
Infected cultured cells*+0
Infected Vero cell supernatant+6.4 × 106 PFU/ml
Infected C6/36 cell supernatant+7.4 × 106 PFU/ml
Mock-infected mosquitoes− (0/3)− (3/3)
Infected mosquitoes (7 dpi)+ (15/15)+ (14/15)
Infected mosquitoes (14 dpi)+ (MD, 9/10; H2O, 10/10)+ (10/10)
Infected mosquito midgut+ (4/5)+ (2/5)
Infected mosquito salivary gland+ (4/5)+ (2/5)
Infected mosquito carcass+ (1/5)+ (1/5)

*Vero cells or C6/36 cells, lysed in water.

†Positive for plaque formation.