Table 1. Quantification of CTCs by microscopy and FACS.

Blood was randomly obtained from 24 patients with MM, and the number of CTCs was detected by fluorescence-activated cell sorting (FACS), gating for live CD45loCD138+CD38+ cells, or by microscopy. In all blood samples, at least 12 CTCs were detected by microscopy. Two time points were analyzed from patient 9.

SamplesNo. of CTCs by FACS (per 100,000)No. of CTCs by microscopy (total)Single-cell sequencing performed
P009—t10>16Yes
P0131>12No
P0161>12No
P0063>12No
P0113>24Yes
P0123>12No
P0044>12No
P0274>24Yes
P0195>12No
P0205>12No
P0225>12No
P0166>12No
P0187>12No
P0027>16Yes
P01413>12No
P02113>24Yes
P02413>23Yes
P03013>24Yes
P01825>12No
P02529>12No
P001102>12Yes
P009—t2162>24Yes
P015204>12No
P03113,708>24Yes