Table 2. Gene expression changes in Tbx5fl/+;R26CreERt2, Pitx2fl/+;R26CreERt2 , and Tbx5fl/+;Pitx2fl/+; R26CreERt2 mouse left atria.

Gene expression TBX5/PITX2 shared targets in left atrial tissue normalized to R26CreERt2 by qRT-PCR, in independent cohorts. Data are means ± SEM (n = 4 R26CreERt2 and n = 6 Tbx5f/f;R26CreERt2 for RNA-seq; n = 4 R26CreERt2 and n = 7 Tbx5fl/fl;R26CreERt2 for qRT-PCR). *P < 0.05 versus R26CreERt2, P < 0.05 versus Tbx5fl/+;R26CreERt2. n = 11 R26CreERt2; n = 15 Tbx5fl/+;R26CreERt2; n = 4 Pitx2fl/+;R26CreERt2; n = 8 Tbx5fl/+;Pitx2fl/+;R26CreERt2. For Tbx5fl/+;R26CreERt2 versus R26CreERt2 Tbx5: P = 1.5 × 10−5; Pitx2c: P = 0.86; Scn5a: P = 0.028; Ryr2: P = 3.98 × 10 −5; Gja1: P = 6.98 × 10−3 and Atp2a2: P = 5.5 × 10−3. For Pitx2fl/+;R26CreERt2 versus R26CreERt2 Pitx2c: P = 0.05; Scn5a: P = 0.03; Tbx5: P = 0.47; Ryr2: P = 0.44; Gja1: P = 0.52; Atp2a2: P = 0.60. For Tbx5fl/+;Pitx2fl/+;R26CreERt2 versus Tbx5fl/+;R26CreERt2 Tbx5: P = 0.56; Scn5a: P = 7.5 × 10−3; Ryr2: P = 0.01; Gja1: P = 0.02; Atp2a2: P = 0.19; Dsp: P = 0.05. Tbx5fl/+;Pitx2fl/+;R26CreERt2 versus R26CreERt2 Scn5a: P = 0.58; Ryr2: P = 0.07; Gja1: P = 0.99; Atp2a2: P = 0.19; Dsp: P = 0.31. By two-tailed t test. Experiments performed in technical triplicate.

Tbx5f/+;R26CreERt2
fold change
Pitx2f/+;R26CreERt2
fold change
Tbx5f/+;Pitx2fl/+;R26CreERt2
fold change
Tbx50.55 ± 0.06*1.11 ± 0.170.64 ± 0.05*
Pitx2c0.91 ± 0.130.45 ± 0.10*0.54 ± 0.22*
Ryr20.59 ± 0.04*1.12 ± 0.150.72 ± 0.14*
Atp2a20.73 ± 0.05*1.10 ± 0.110.86 ± 0.08
Dsp1.07 ± 0.070.88 ± 0.141.31 ± 0.21
Gja10.70 ± 0.04*0.95 ± 0.060.98 ± 0.03
Scn5a0.85 ± 0.08*1.95 ± 0.10*1.18 ± 0.23