Gene expression of ion channels implicated in AF and of Tbx5/Pitx2 cotargets in left atrial tissue from Tbx5f/f;R26CreERt2 adult mutant mice normalized to R26CreERt2 by RNA-seq or qRT-PCR, in independent cohorts. Data are means ± SEM (n = 4 R26CreERt2 and n = 6 Tbx5f/f;R26CreERt2 for RNA-seq; n = 4 R26CreERt2 and n = 7 Tbx5fl/fl;R26CreERt2 for qRT-PCR). Nonsignificant changes in the RNA-seq were not validated by real time, with the exception of Pitx2c, which was just above significance by RNA-seq.
| Gene | Tbx5fl/fl;R26CreERt2 versus R26CreERt2 fold change by RNA-seq | q (RNA-seq) | Tbx5f/f;R26CreERt2 versus R26CreERt2 fold change by qRT-PCR | P (qRT-PCR) |
| Pitx2c | 0.53 | 0.07 | 0.22 ± 0.09 | 0.03 |
| Gja5 | 0.05 | 3 × 10−4 | 0.23 ± 0.05 | 2.49 × 10−6 |
| Gja1 | 0.24 | 3 × 10−4 | 0.30 ± 0.04 | 1.06 × 10−5 |
| Dsp | 0.36 | 3 × 10−4 | 0.44 ± 0.05 | 1.99 × 10−8 |
| Scn5a | 0.30 | 3 × 10−4 | 0.34 ± 0.07 | 1.06 × 10−5 |
| Ryr2 | 0.22 | 3 × 10−4 | 0.42 ± 0.07 | 2.14 × 10−6 |
| Atp2a2 | 0.42 | 3 × 10−4 | 0.42 ± 0.17 | 0.002 |
| Sln | 0.41 | 3 × 10−4 | 0.52 ± 0.05 | 1.2 × 10−4 |
| Kcnd3 | 0.67 | 7 × 10−3 | 0.25 ± 0.17 | 0.002 |
| Kcnj3 | 0.14 | 3 × 10−4 | 0.12 ± 0.01 | 9.56 × 10−12 |
| Kcnn2 | 0.77 | 0.10 | 0.33 ± 0.08 | 4.83 × 10−5 |
| Kcnj8 | 0.67 | 7 × 10−3 | 0.38 ± 0.04 | 3 × 10−6 |
| Kcna5 | 0.78 | 0.04 | 0.42 ± 0.13 | 4 × 10−4 |
| Kcnj2 | 0.60 | 3 × 10−4 | 0.42 ± 0.09 | 1.4 × 10−4 |
| Kcnj5 | 0.21 | 3 × 10−4 | 0.49 ± 0.2 | 4.7 × 10−5 |
| Abcc9 | 0.58 | 3 × 10−4 | 0.63 ± 0.04 | 1.7 × 10−5 |
| Cacna1c | 0.95 | 0.88 | ||
| Camk2a/b/d/g | 0.24/0.83/0.59/1.25 | 0.11/1/0.2/1 | ||
| Slc8a1/2/3 | 0.81/3.08/0.61 | 0.18/1/1 | ||
| Calm2/3 | 1.27/1.44 | 0.03/4 × 10−4 |