Table 1. Gene expression changes in Tbx5fl/fl;R26CreERt2 mouse left atria.

Gene expression of ion channels implicated in AF and of Tbx5/Pitx2 cotargets in left atrial tissue from Tbx5f/f;R26CreERt2 adult mutant mice normalized to R26CreERt2 by RNA-seq or qRT-PCR, in independent cohorts. Data are means ± SEM (n = 4 R26CreERt2 and n = 6 Tbx5f/f;R26CreERt2 for RNA-seq; n = 4 R26CreERt2 and n = 7 Tbx5fl/fl;R26CreERt2 for qRT-PCR). Nonsignificant changes in the RNA-seq were not validated by real time, with the exception of Pitx2c, which was just above significance by RNA-seq.

GeneTbx5fl/fl;R26CreERt2 versus R26CreERt2 fold
change by RNA-seq
q
(RNA-seq)
Tbx5f/f;R26CreERt2 versus R26CreERt2 fold
change by qRT-PCR
P (qRT-PCR)
Pitx2c0.530.070.22 ± 0.090.03
Gja50.053 × 10−40.23 ± 0.052.49 × 10−6
Gja10.243 × 10−40.30 ± 0.041.06 × 10−5
Dsp0.363 × 10−40.44 ± 0.051.99 × 10−8
Scn5a0.303 × 10−40.34 ± 0.071.06 × 10−5
Ryr20.223 × 10−40.42 ± 0.072.14 × 10−6
Atp2a20.423 × 10−40.42 ± 0.170.002
Sln0.413 × 10−40.52 ± 0.051.2 × 10−4
Kcnd30.677 × 10−30.25 ± 0.170.002
Kcnj30.143 × 10−40.12 ± 0.019.56 × 10−12
Kcnn20.770.100.33 ± 0.084.83 × 10−5
Kcnj80.677 × 10−30.38 ± 0.043 × 10−6
Kcna50.780.040.42 ± 0.134 × 10−4
Kcnj20.603 × 10−40.42 ± 0.091.4 × 10−4
Kcnj50.213 × 10−40.49 ± 0.24.7 × 10−5
Abcc90.583 × 10−40.63 ± 0.041.7 × 10−5
Cacna1c0.950.88
Camk2a/b/d/g0.24/0.83/0.59/1.250.11/1/0.2/1
Slc8a1/2/30.81/3.08/0.610.18/1/1
Calm2/31.27/1.440.03/4 × 10−4