Table 1. SRE criteria for natural conception applied to differential germ cell markers.

Differentiated germ cell markers provided in the technical comment of Cappallo-Obermann and Spiess (3) were examined. The first SRE criterion for natural conception required that a sperm element exceed the 99th percentile rank on average across seven fertile control samples. From the 28 sperm elements examined, only 13 met the first criterion (marked with an asterisk, percentile rank average). Some elements, such as ODF1 and OAZ3, that did not satisfy the first criterion are moderately abundant (95th to 98th percentile rank). Other elements, such as PFN4 and ACRV1, are absent in the majority of control samples (zeroth percentile rank). These spermatid-specific RNAs are not present in mature spermatozoa and accordingly were not considered. If the first criterion was satisfied, the second outlier criterion was applied. This discarded those elements that did not satisfy the IQR rule. Of the 13 elements exceeding the 99th percentile range on average across the control samples, only 6 satisfied the IQR rule (marked with a dagger sign) and were retained as SREs from four genes: TNP1, LELP1, SMCP, and OAZ3.

Transcript
name
S1S2S3S4S5S6S7Percentile rank
average
Outlier
(<Q1–1.5 × IQR)
Outliers in
control
samples
PRM1*0.9999960.9999640.9999800.9999780.9999430.9998670.9999930.9999520.9998911
PRM1*0.9999890.9999280.999960.9999640.9999250.9997490.9999820.9999170.9998671
PRM2*0.9999780.9999860.9999710.9999960.9999280.9999640.9999750.9999670.9999481
PRM2*0.9999390.9999030.999940.9999680.9997670.9999210.9999570.9999100.9998671
ODF10.9898710.9734250.9708510.9743970.8873960.9615440.9867450.9561866
ODF10.9925160.9880080.9890490.9938190.9801010.9862490.9899140.9878264
TNP1*0.9999640.9992640.9999350.9999350.9991600.9994440.9999530.9996850.9987080
TNP1*0.9999930.9998960.9999820.9999860.9997700.9998960.9999780.9999220.9997670
LELP1*0.9996160.9988940.9993000.9996880.9973370.9987260.9991740.9988450.9978651
LELP1*0.9995190.9987720.9992430.9996980.9983670.9991420.9988660.9990630.9983010
SMCP*0.9996770.9998640.9998640.9999390.9996980.9998100.9999500.9998520.9996170
SMCP*0.9994360.9995190.9995940.9998890.9994900.9996910.9998380.9997000.9992280
OAZ30.9793720.9771540.9825920.9921140.8350350.9664260.9820790.951649
OAZ3*0.9974730.9971970.9986900.9992100.9947420.9972580.9988510.9977500.9948691
OAZ3*0.9973010.9942000.9982700.9986860.9941890.9974700.9976560.9972540.9962700
OAZ3*0.9935430.9835720.9926890.9973470.9747920.9929830.9932340.9902090.9918722
OAZ30.8331900.9672330.9710130.9875090.9152530.9862960.9823760.968489
OAZ30.9848500.9790530.9787910.9966550.9457220.9899320.9918380.980588
PFN40.0000000.0000000.0000000.7489350.0000000.0000000.0000000.149787
PFN40.0000000.0000000.0000000.7836110.0000000.0000000.0000000.156722
PFN40.0000000.0000000.0000000.0000000.0000000.0000000.0000000.000000
PFN40.0000000.0000000.0000000.0000000.0000000.0000000.0000000.000000
PFN40.0000000.0000000.0000000.8493310.0000000.0000000.0000000.169866
ACRV10.0000000.8058970.0000000.7351380.0000000.0000000.0000000.147028
ACRV10.0000000.8899410.9424380.9173670.5778610.0000000.0000000.487533
ACRV10.7705570.7512570.8807420.8821490.6094180.0000000.0000000.474462
ACRV10.0000000.0000000.0000000.8699020.0000000.7754740.0000000.329075
ACRV10.0000000.0000000.0000000.8188910.0000000.7027050.0000000.304319