Table 1. Vasodilation of strial capillaries by LPS.

In basal coils, strial capillary diameters in P6 pups were significantly larger than those in DPBS-treated C57BL/6 adult mice (****P < 0.0001). LPS dilated a subset (45%) of strial capillaries in adult mice. The population of capillary diameters in LPS-treated C57BL/6 mice was significantly greater compared to that in DPBS-treated C57BL/6 mice (P < 0.0001). LPS also dilated a subset (50%) of strial capillaries in C3H/HeOuJ mice. In TLR4-hyporesponsive C3H/HeJ mice, LPS dilated a smaller subset (22%) of strial capillaries. The population of capillary diameters in LPS-treated C3H/HeJ mice was significantly greater compared to that in DPBS-treated C3H/HeJ mice (P < 0.0001). The population of capillary diameters in LPS-treated C3H/HeJ mice was significantly smaller compared to that in LPS-treated C3H/HeOuJ mice (P < 0.0001, one-way ANOVA with Tukey post hoc tests). In apical coils, LPS dilated a subset (45%) of strial capillaries in C57BL/6 mice. The population of apical strial capillary diameters in LPS-treated C57BL/6 mice was significantly greater compared to that in DPBS-treated C57BL/6 adult mice (P < 0.0001). A Gaussian regression curve fit was applied to obtain the peak means of the bimodal distributions in Fig. 3.

MouseLPSMean
diameter
(±SD)
in μm
Mean
bimodal
diameter
(±SD) in μm
[% of n]
n
(number
of mice)
Significance
(versus
in-strain
DPBS-treated
mice)
Basal coils
C57BL/6
P6 pups
13.8 (±1.5)300 (3)****
C57BL/6
adults
5.1 (±1.0)300 (3)
C57BL/6
adults
+5.3 (±0.9)8.6 (±1.1)
[45.3%]
300 (3)****
C3H/HeOuJ
adults
5.3 (±0.8)480 (4)
C3H/HeOuJ
adults
+5.7 (±0.8)8.6 (±0.9)
[49.7%]
480 (4)****
C3H/HeJ
adults
5.3 (±0.9)480 (4)
C3H/HeJ
adults
+5.4 (±0.8)8.1 (±0.9)
[22.1%]
480 (4)****
Apical coils
C57BL/6
adults
5.0 (±0.7)480 (4)
C57BL/6
adults
+5.6 (±0.6)8.1 (±1.0)
[59.8%]
480 (4)****