Table 3. Evaluation of in vitro generated DSM265 Dd2–resistant parasites.

Fold change in parentheses. PfDHODH data are IC50 values determined on the recombinant wild-type (wt) or G181C mutant P. falciparum enzymes. All data were collected with a minimum of three technical replicates and then used for a global fit. Error analysis is provided in table S5. N/A, not applicable.

Clone numberInoculumDSM265 selection
concentration (μg/ml)
DSM265 Dd2 EC50
(μg/ml)
Gene copy
number
DHODH
mutation
PfDHODH IC50
(μg/ml)
Dd2/wtN/AN/A0.00221.0N/A0.0096
PR.Cl12 × 1070.00830.0083 (3.8)2.7NoneN/A
PR.Cl22 × 1070.00830.0099 (4.5)2.3NoneN/A
DF.R10 Clb2 × 1060.00950.058 (26)1.1G181C0.120 (12)