Table 1. Bacterial strains and mammalian cell types used in this study.

AHL, N-(3-oxohexanoyl)-l-homoserine lactone; GEMM, genetically engineered mouse model.

LabelBacterial strain or
mammalian cell
Genomic/plasmidsUse(s)
PROP-ZEcNluxCDABE (genomic),
IPTG-inducible lacZ (stabilized plasmid)
Bioluminescent imaging
of bacteria, urine
diagnostic assay
PROP-LucEcNluxCDABE (genomic),
luxCDABE (plasmid)
Bioluminescent imaging
of bacteria
PROPi-LucEcNAHL-inducible
luxCDABE (plasmid)
Bioluminescent imaging
of bacteria
Non-lacZMach OnelacZΔM15 (genomic mutant)Non-lacZ control for urine
diagnostic assay
MC26-LucFMetastatic colorectal
mouse cell line
Firefly luciferase (genomic)Subcutaneous xenografts and
liver metastasis models
LS174T-LucFHuman colorectal
adenocarcinoma cell line
Firefly luciferase (genomic)Subcutaneous xenograft models
393M1-LucFGEMM lung mouse cell lineFirefly luciferase (genomic)Subcutaneous xenografts and
liver metastasis models