Table 1. Assessment of the differences in tissue infection rates in wMel versus wild-type mosquitoes using the sign test.

The experimental data were treated as pairs of binomial observations corresponding to the proportions infected of the wMel and wild-type mosquito groups fed on a particular blood sample, which were sampled on a specific day. The table rows present the number of observation pairs for which the proportion of wMel mosquitoes infected was less than, equal to, or greater than the proportion of wild-type mosquitoes infected for different data subsets. Subsets are shown that stratify the observations by tissue type, DENV serotype, and the day after infection that mosquitoes were assayed. The two-sided P value is given, with P values <0.05 shown in italics.

TissueSerotypeDayn pairs
where
pwMel < pWT
n pairs
where
pwMel = pWT
n pairs
where
pwMel > pWT
P value
(for accepting
pwMel = pWT)
AllAll4155140.0004
All73931
All10162060.053
All14161830.0044
AbdomenAll186820.29
DENV1All172550.017
DENV2All61051
DENV3All6420.29
DENV4All121620.013
AllAll571413< 0.0001
All74520.69
All102272< 0.0001
All1422260.0037
Saliva (conditional)All189030.15
DENV1All304110.0043
DENV2All11410.0063
DENV3All6210.13
DENV4All10400.002
AllAll591610< 0.0001
All74620.69
All102272< 0.0001
All142433< 0.0001
Saliva (unconditional)All189030.15
DENV1All31590.0007
DENV2All11410.0063
DENV3All7300.0156
DENV4All10400.002