Table 1 Clinical description and outcome of NiV-challenged AGMs.

APTT, activated partial thromboplastin time; AST, aspartate aminotransferase; BUN, blood urea nitrogen; CRE, creatinine; CRP, C-reactive protein; GGT, γ-glutamyltransferase; PT, partial thromboplastin time.

Subject no.GenderGroupClinical illnessClinical and gross pathology
R272FemaleControlDepression (d8); lethargy (d8); loss of appetite
(d8); dyspnea (d8); open-mouth breathing
(d8); dehydration (d7, d8). Animal expired
on d8.
Thrombocytopenia (d7–d8); lymphopenia (d7);
hypoalbuminemia (d8); >10-fold ↑ in AST (d8);
5-fold ↑ in BUN (d8); >3-fold ↑ in CRE (d8); >10-fold ↑
in CRP (d7–d8); >5-fold ↑ in APTT (d8); >2-fold ↑
in fibrinogen (d8); excess blood-tinged pleural fluid;
severely inflated, enlarged lungs with multifocal
areas of congestion and hemorrhage; dark liver.
V753FemaleControlDepression (d8–d10); lethargy (d8–d10); loss
of appetite (d8–d10); severe dyspnea
(d9–d10); dehydration (d7–d10); muscle
twitches (d10). Animal euthanized on d10.
Mild thrombocytopenia (d3); >3-fold ↑ in CRP (d3, d5,
d7, d10); >2-fold ↑ in
PT (d10); >2-fold ↑ in fibrinogen (d3, d5, d7); excess blood-tinged pleural
fluid; moderately inflated, enlarged lungs with
multifocal areas of congestion and hemorrhage
particularly on the lower lobes.
R318FemaleControlFever (d7); depression (d8–d9); lethargy
(d8–d9); loss of appetite (d8–d9); dyspnea
(d9); dehydration (d7–d9); left hindlimb
paresis (d9). Animal euthanized on d9.
Thrombocytopenia (d5); lymphopenia (d3, d5, d7);
2-fold ↑ in APTT (d9); excess blood-tinged pleural
fluid; moderately inflated, enlarged lungs with
multifocal areas of congestion and hemorrhage
particularly on the right lobes and the lower
left lobe; mild splenomegaly.
O201216MaleControlFever (d10); depression (d10); lethargy (d10);
loss of appetite (d10); dyspnea (d7–d10);
open-mouth and abdominal breathing
(d7–d10); muscle twitches (d10); paresis of
both hindlimbs (d10). Animal euthanized
on d10.
Thrombocytopenia (d7, d10); lymphopenia (d7);
>3-fold ↑ in GGT (d10); >7-fold ↑ in CRP (d10);
>2-fold ↑ in fibrinogen (d10); excess blood-tinged
pleural fluid; inflated, enlarged lungs with multifocal
areas of congestion and hemorrhage particularly of
the lower right lobe; accumulation of fluid on
pericardial cavity; spleen congested and friable;
petechial hemorrhages on mucosal surface of
urinary bladder; congestion of brain with possible
meningeal hemorrhage.
R300FemaleD1/D3NoneNone
Y083FemaleD1/D3NoneNone
R429FemaleD1/D3NoneNone
O7245MaleD1/D3NoneNone
R183FemaleD3/D5Fever (d15)>2-fold increase CRP (d5)
Y809MaleD3/D5NoneThrombocytopenia (d7)
R145FemaleD3/D5None>4-fold ↑ in CRP (d5); >2-fold ↑ in fibrinogen (d5)
O7823FemaleD3/D5NoneNone
O7383MaleD5/D7Fever (d7); mild depression (d10–d13);
abdominal breathing (d11–d16);
muscle twitches on right ear (d11–d14)
Thrombocytopenia (d10); mild thrombocytopenia
(d7); >2-fold ↑ in CRP (d7)
O7818FemaleD5/D7Fever (d7); anorexia (d8–d12); mild
dyspnea (d8–d9)
Mild thrombocytopenia (d7); >7-fold ↑ in CRP (d7);
>2-fold ↑ in CRP (d10); >2-fold ↑ in fibrinogen (d10)
O918745MaleD5/D7NoneMild thrombocytopenia (d7, d10)
R198FemaleD5/D7NoneThrombocytopenia (d7); >5-fold ↑ in CRP (d7);
>2-fold ↑ in fibrinogen (d7)