Table 1 CTC selection and culture.

Peripheral blood samples from a population of 38 patients with invasive breast cancer were initially analyzed by CellSearch (EpCAM+ CTCs). Undetectable CTC levels (CTC = 0) were found in 15 patients’ samples. Analyses of these patient PBMCs showed eight patients to have EGFR gain/ALDH+/HPSE+ CACCs. PBMCs isolated from these patients underwent multiparametric FACS analysis (CD45/ALDH1+/EpCAM selection). Successful FACS selection for potential CTCs was achieved in three patients. Only EpCAM CACCs could survive and grow in culture to establish CTC lines CTC-1, CTC-2, and CTC-3 (in bold).

Patient IDCTC detection
by CellSearch
EGFR
gain/ALDH1+/HPSE+
CTC capture by
FACS and
viable culture
n = 38n = 15n = 23n = 8n = 8
1Undetectable+Nonviable CTC
2+
3+
4+
5Undetectable
6Undetectable+Nonviable CTC
7+
8+
9Undetectable
10Undetectable+Nonviable CTC
11Undetectable
12+
13+
14Undetectable
15+
16Undetectable+Viable CTC (CTC-1)
17+
18Undetectable
19+
20Undetectable+Nonviable CTC
21+
22Undetectable+Viable CTC (CTC-2)
23Undetectable+Viable CTC (CTC-3)
24+
25+
26+
27Undetectable
28+
29+
30+
31+
32Undetectable+Nonviable CTC
33+
34+
35Undetectable
36+
37+
38+