Table 1

Summary of next-generation sequencing analyses performed. Data were obtained using next-generation sequencing analyses performed on Illumina HiSeq instruments using 50-bp PE reads. Distinct paired reads correspond to read pairs having unique genomic start sites. Sequence coverage indicates average number of reads per base per haploid genome. Physical coverage indicates average number of paired reads spanning any base in a haploid genome assuming a 165-bp fragment size.

Sample
name
PatientdiagnosisSample
origin
Total bases
sequenced
Total distinct
paired reads
Sequence
coverage
Physical
coverage
CRC11Colorectal cancerPlasma24,728,144,682216,092,2048.211.9
CRC12Colorectal cancerPlasma25,707,029,400221,881,4998.612.2
CRC12-PTColorectal cancerTumor14,984,097,228113,414,8595.06.2
CRC13Colorectal cancerPlasma24,033,905,652206,926,8438.011.4
CRC14Colorectal cancerPlasma23,774,411,124201,571,4267.911.1
CRC14-0Colorectal cancerPlasma3,113,755,96017,297,6311.01.0
CRC14-4Colorectal cancerPlasma3,755,921,75021,193,1761.31.2
CRC14-PTColorectal cancerTumor7,156,542,10555,882,3872.43.1
CRC15Colorectal cancerPlasma34,216,224,513299,895,77911.416.5
CRC15-PTColorectal cancerTumor12,466,375,200102,193,6304.25.6
CRC16Colorectal cancerPlasma32,670,584,037284,131,68410.915.6
CRC16-PTColorectal cancerTumor6,199,959,15041,700,4932.12.3
CRC17Colorectal cancerPlasma33,060,006,522286,524,06711.015.8
BR1Breast cancerPlasma34,918,959,429304,798,84011.616.8
BR2Breast cancerPlasma30,171,911,865240,263,50510.113.2
BR3Breast cancerPlasma31,294,294,671259,443,65910.414.3
N1NormalPlasma26,918,560,359231,520,3149.012.7
N2NormalPlasma25,928,759,499224,708,0178.612.4
N3NormalPlasma21,331,401,576161,874,9347.18.9
N4NormalPlasma25,735,971,696223,342,3098.612.3
N5NormalPlasma33,535,967,796288,318,72111.215.9
N6NormalPlasma32,892,667,872285,785,10711.015.7
N7NormalPlasma27,558,615,816226,653,0769.212.5
N8NormalPlasma32,472,392,886279,224,46910.815.4
N9NormalPlasma30,058,183,548257,431,31210.014.2
N10NormalPlasma33,068,060,850287,180,67511.015.8