Table 3

Specific expression of IFN-γ in B7-H1+ melanomas. IFN-γ was detected by qRT-PCR as described in Materials and Methods. These results are representative of two separate experiments. UD, undetectable (CT greater than 45 cycles); NA, not applicable.

Patient numberTissue sourceCT
(mean)*
SEMCT
(mean)
SEM
B7-H1 tumors
1Lymph node metastasisUDNA33.190.45
2Small bowel metastasisUD§NA34.460.38
3Lymph node metastasisUDNA32.930.11
4Lymph node metastasisUDNA35.530.28
5Cutaneous primary lesionUDNA36.110.13
B7-H1+ tumors
6Lymph node metastasis34.670.4932.410.16
7Lymph node metastasis35.000.3233.550.36
8Lung metastasis33.150.3031.910.78
9Lung metastasis31.800.0728.430.02
10Lymph node metastasis35.170.2232.510.22
Controls
NAPBMCs33.120.3528.550.66
NAActivated CD3+ T cells23.600.0828.330.11
NA1359-melUDNAUDNA

*Lower values reflect greater expression.

†SEM of triplicate samples.

‡One of three triplicate reactions for patient 1 tumor had CT = 41.7, and two of three were undetectable and average undetectable.

§One of three triplicate reactions for patient 2 tumor had CT = 43.1, and two of three were undetectable and average undetectable.