Table 1

Kinetic parameters of ALK-TKD. Data are shown as means ± SEM of at least three independent experiments. Details of analysis are described in the Supplementary Methods. P values quoted in the text were determined with an unpaired t test.

Kinasekcat
(min−1)
Km, peptide
(mM)
kcat/Km, peptide
(min−1 mM−1)
Km, ATP*
(mM)
kcat/Km, ATP
(min−1 mM−1)
Wild type9.32 ± 0.852.88 ± 0.423.41 ± 0.440.134 ± 0.00769.7
R1275Q119 ± 132.56 ± 0.3246.8 ± 1.90.326 ± 0.033364
F1174L365 ± 619.18 ± 1.4339.7 ± 2.80.127 ± 0.0112870
Phospho–wild type424 ± 631.80 ± 0.17237 ± 350.159 ± 0.0122660
Phospho-R1275Q347 ± 151.39 ± 0.10252 ± 240.248 ± 0.0151400
Phospho-F1174L436 ± 511.25 ± 0.20357 ± 250.109 ± 0.0013980

*Determined at 1 mM peptide.