Table 2

High-resolution characterization of residual tumor burden and normal B cells in UPN 01 and 03. Analyses were performed on DNA isolated from whole blood by high-throughput IgH immune profiling (Adaptive TcR Corp.). PB, peripheral blood; BM, bone marrow.

Patient
(UPN)
TissueTime
point
Amount of DNA
in PCR (ng)
Cell equivalentsTotal productive
sequence reads
Total productive
unique B cell
clonotypes
CLL IGH@
CDR3
clone reads
Clone frequency
(% productives)
01PBPreinfusion1000158,730184,78624184,25699.713
01PBDay −11000158,730408,57948407,59299.758
01PBDay +281000158,7300000.000
01PBDay +17650079,365285,3057,36200.000
01BMDay +281000158,7300000.000
01BMDay +1761000158,730202,5354,45100.000
03PBPreinfusion2000317,46022,074,91258,23419,948,50890.367
03PBDay −138661,2701,385,3404,5441,231,01888.860
03PBDay +311000158,7300000.000
03PBDay +1762000317,4600000.000
03BMDay +311750277,7780000.000
03BMDay +1761000158,7300000.000