Table 1

454 sequencing of EGFR exon 20. About 100,000 454 reads per sample were generated from PCR products generated with EGFR exon 20 (ex20)–spanning primers. All tumors were from treatment-naïve patients. TKI-R-1 and TKI-R-2 were run as positive control samples. 1°mutn, primary EGFR mutation, as assessed by direct sequencing.

SampleStage1°mutn454 ex20 T790M (%)
Lung TKI-R-1IV19 DELY (59%)
Lung TKI-R-2IVL858RY (1.07%)
H3255Cell LineL858RN
PC-9Cell Line19 DELN
130TIA19 DELN
169TIA19 DELN
230TIA19 DELN
474TIA19 DELN
631TIA19 DELN
20TIB19 DELN
734TIB19 DELN
739TIB19 DELN
388TIIIA19 DELN
3TIAL858RN
5TIAL858RN
485TIAL858RN
570TIBL858RN
685TIBL858RN
25TIBL858RN
166TIBL858RN