Table 4

Coincident IgH sequences in peripheral blood of healthy donors of various ages. Peripheral blood samples from 23 healthy donors of ages ranging from 19 to 79 years were analyzed by deep sequencing IgH rearrangements in six replicates from each sample. The number of distinct sequences detected in more than one replicate (termed coincident sequences) from each individual is tabulated below. Sequences from different replicates were considered to be coincident sequences if they shared the same V, D, and J segment usage as well as the same V-D and D-J junctional nucleotide sequences. Calculation of the minimum IgH repertoire diversity in each patient, as indicated by the number of coincident sequences detected, is described in the Supplementary Material.

AgeTotal sequencesCoincidencesMinimum diversity
23456
1919,3682200002,136,616
2012,598610200704,883
236,9641100001,133,759
256,5221000001,328,380
314,086101100474,366
326,112900001,328,380
355,358400001,860,053
375,253411001,973,903
382,17318210070,876
424,094110000381,515
442,24930000438,241
456,781652200325,619
457,6971200001,409,687
506,841610001,718,401
5410,8221310013,369,228
553,42670000513,469
605,17383000704,883
615,092100006,349,446
687,0281112011,897,254
705,5521000001,276,797
757,064501003,303,164
785,895400003,051,613
797,1271100001,587,537