Table 3

Coincident sequences in a healthy donor’s peripheral blood at two time points. IgH rearrangements from peripheral blood mononuclear cells of a healthy blood donor were PCR amplified in multiple independent replicate PCR reactions and sequenced. The table shows the number of identical sequences detected in more than one replicate (termed coincident sequences). Blood samples from two time points separated by 14 months were analyzed. Sequences from different replicates were considered to be coincident sequences if they shared the same V, D, and J segment usage as well as the same V-D and D-J junctional nucleotide sequences. T1, initial time point; T2, second time point 14 months later; r1 through r7, replicates 1 through 7.

T1r2T1r3T1r4T1r5T1r6T2r1T2r2T2r3T2r4T2r5T2r6T2r7
T1r1221112120221
T1r211043001010
T1r30200010010
T1r4120011031
T1r511001022
T1r60000021
T2r1011111
T2r202201
T2r31202
T2r4202
T2r510
T2r65