RT Journal Article SR Electronic T1 Elite control of HIV is associated with distinct functional and transcriptional signatures in lymphoid tissue CD8+ T cells JF Science Translational Medicine FD American Association for the Advancement of Science SP eaax4077 DO 10.1126/scitranslmed.aax4077 VO 11 IS 523 A1 Nguyen, Son A1 Deleage, Claire A1 Darko, Samuel A1 Ransier, Amy A1 Truong, Duc P. A1 Agarwal, Divyansh A1 Japp, Alberto Sada A1 Wu, Vincent H. A1 Kuri-Cervantes, Leticia A1 Abdel-Mohsen, Mohamed A1 Del Rio Estrada, Perla M. A1 Ablanedo-Terrazas, Yuria A1 Gostick, Emma A1 Hoxie, James A. A1 Zhang, Nancy R. A1 Naji, Ali A1 Reyes-Terán, Gustavo A1 Estes, Jacob D. A1 Price, David A. A1 Douek, Daniel C. A1 Deeks, Steven G. A1 Buggert, Marcus A1 Betts, Michael R. YR 2019 UL http://stm.sciencemag.org/content/11/523/eaax4077.abstract AB CD8+ T cells are often referred to as cytotoxic lymphocytes, but their functions extend beyond lysis of targets. Moreover, resident CD8+ T cells are not identical to their better studied circulating counterparts. To better understand functions of highly effective lymphoid CD8+ T cells, Nguyen et al. sampled lymph nodes from HIV elite controllers. Compared with cells from people with progressive disease, the elite controller cells had a distinct transcriptional profile and were able to suppress viral replication in the absence of cytolysis. CD8+ T cells from elite controllers translated proteins more efficiently, which could contribute to viral control. These results elucidate natural mechanisms of HIV control that could be informative for cure efforts or vaccine design.The functional properties of circulating CD8+ T cells have been associated with immune control of HIV. However, viral replication occurs predominantly in secondary lymphoid tissues, such as lymph nodes (LNs). We used an integrated single-cell approach to characterize effective HIV-specific CD8+ T cell responses in the LNs of elite controllers (ECs), defined as individuals who suppress viral replication in the absence of antiretroviral therapy (ART). Higher frequencies of total memory and follicle-homing HIV-specific CD8+ T cells were detected in the LNs of ECs compared with the LNs of chronic progressors (CPs) who were not receiving ART. Moreover, HIV-specific CD8+ T cells potently suppressed viral replication without demonstrable cytolytic activity in the LNs of ECs, which harbored substantially lower amounts of CD4+ T cell–associated HIV DNA and RNA compared with the LNs of CPs. Single-cell RNA sequencing analyses further revealed a distinct transcriptional signature among HIV-specific CD8+ T cells from the LNs of ECs, typified by the down-regulation of inhibitory receptors and cytolytic molecules and the up-regulation of multiple cytokines, predicted secreted factors, and components of the protein translation machinery. Collectively, these results provide a mechanistic framework to expedite the identification of novel antiviral factors, highlighting a potential role for the localized deployment of noncytolytic functions as a determinant of immune efficacy against HIV.