RT Journal Article
SR Electronic
T1 Two-mAb cocktail protects macaques against the Makona variant of Ebola virus
JF Science Translational Medicine
FD American Association for the Advancement of Science
SP 329ra33
OP 329ra33
DO 10.1126/scitranslmed.aad9875
VO 8
IS 329
A1 Qiu, Xiangguo
A1 Audet, Jonathan
A1 Lv, Ming
A1 He, Shihua
A1 Wong, Gary
A1 Wei, Haiyan
A1 Luo, Longlong
A1 Fernando, Lisa
A1 Kroeker, Andrea
A1 Bovendo, Hugues Fausther
A1 Bello, Alexander
A1 Li, Feng
A1 Ye, Pei
A1 Jacobs, Michael
A1 Ippolito, Giuseppe
A1 Saphire, Erica Ollmann
A1 Bi, Shengli
A1 Shen, Beifen
A1 Gao, George F.
A1 Zeitlin, Larry
A1 Feng, Jiannan
A1 Zhang, Boyan
A1 Kobinger, Gary P.
YR 2016
UL http://stm.sciencemag.org/content/8/329/329ra33.abstract
AB Antibody cocktails are an appealing therapeutic option for emerging infections such as the recent Ebola virus outbreak in West Africa because of their scalability and specificity. Qiu et al. report that the antibody cocktail used in Ebola virus–infected patients can be further simplified to only two antibodies and that these antibodies can be produced in engineered Chinese hamster ovary cells. This cocktail protected nonhuman primates against the virus responsible for the 2014–2015 outbreak up to 3 days after exposure. Combining these antibodies with those specific for other strains may lead to a broad ebolavirus therapy.The 2014–2015 Ebola virus (EBOV) outbreak in West Africa highlighted the urgent need for specific therapeutic interventions for infected patients. The human-mouse chimeric monoclonal antibody (mAb) cocktail ZMapp, previously shown to be efficacious in EBOV (variant Kikwit) lethally infected nonhuman primates (NHPs) when administration was initiated up to 5 days, was used in some patients during the outbreak. We show that a two-antibody cocktail, MIL77E, is fully protective in NHPs when administered at 50 mg/kg 3 days after challenge with a lethal dose of EBOV variant Makona, the virus responsible for the ongoing 2014–2015 outbreak, whereas a similar formulation of ZMapp protected two of three NHPs. The chimeric MIL77E mAb cocktail is produced in engineered Chinese hamster ovary cells and is based on mAbs c13C6 and c2G4 from ZMapp. The use of only two antibodies in MIL77E opens the door to a pan-ebolavirus cocktail.