PT - JOURNAL ARTICLE AU - Guha, Rajan AU - Gupta, Deepika AU - Rastogi, Ruchir AU - Vikram, Rajagopal AU - Krishnamurthy, Ganga AU - Bimal, Sanjiva AU - Roy, Syamal AU - Mukhopadhyay, Amitabha TI - Vaccination with <em>Leishmania</em> Hemoglobin Receptor–Encoding DNA Protects Against Visceral Leishmaniasis AID - 10.1126/scitranslmed.3006406 DP - 2013 Sep 11 TA - Science Translational Medicine PG - 202ra121--202ra121 VI - 5 IP - 202 4099 - http://stm.sciencemag.org/content/5/202/202ra121.short 4100 - http://stm.sciencemag.org/content/5/202/202ra121.full AB - Leishmaniasis is a severe infectious disease. Drugs used for leishmaniasis are very toxic, and no vaccine is available. We found that the hemoglobin receptor (HbR) of Leishmania was conserved across various strains of Leishmania, and anti-HbR antibody could be detected in kala-azar patients’ sera. Our results showed that immunization with HbR-DNA induces complete protection against virulent Leishmania donovani infection in both BALB/c mice and hamsters. Moreover, HbR-DNA immunization stimulated the production of protective cytokines like interferon-γ (IFN-γ), interleukin-12 (IL-12), and tumor necrosis factor–α (TNF-α) with concomitant down-regulation of disease-promoting cytokines like IL-10 and IL-4. HbR-DNA vaccination also induced a protective response by generating multifunctional CD4+ and CD8+ T cells. All HbR-DNA–vaccinated hamsters showed sterile protection and survived during an experimental period of 8 months. These findings demonstrate the potential of HbR as a vaccine candidate against visceral leishmaniasis.