Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Clustering of ARH-ME MC3R neurons.
  • Fig. S2. Expression pattern of neuropeptides in ARH-ME MC3R neuronal subclusters.
  • Fig. S3. Mc3r coexpression in several orexigenic cell types in the ARH.
  • Fig. S4. MC3R KO mice consume less food during NSF assay.
  • Fig. S5. Female MC3R KO mice exhibit defective fast-induced refeeding.
  • Fig. S6. MC3R KO mice exhibit increased anxiety-related behavior.
  • Fig. S7. MC3R TB/TB mice show elevated anxiety-like behavior in the EPM test.
  • Fig. S8. DREADD-mediated activation of ARH MC3R neurons increases feeding in male and female mice.
  • Fig. S9. CNO administration does not affect food intake in WT mice.
  • Fig. S10. Manipulation of ARH MC3R neurons does not affect locomotion in the EPM and OFT.
  • Fig. S11. Paired analysis of feeding effects of MC3R-specific compounds.
  • Fig. S12. Pharmacological manipulation of MC3R affects feeding in male and female mice.
  • Fig. S13. MC3R stimulation does not alter energy expenditure, movement, or nutrient partitioning.
  • Fig. S14. MC3R stimulation does not alter heart rate, blood pressure, or activity.
  • Fig. S15. Inhibition of MC3R enhances pharmacological anorexia.
  • Fig. S16. MC3R antagonism does not affect anxiety-like behavior or food avoidance in the NSF test.
  • Fig. S17. Effects of MC3R compound administration in WT and AgRP-MC3R KI mice.
  • Fig. S18. Model for MC3R-mediated effects on feeding.
  • Fig. S19. MC3R circuit model.
  • Table S1. Sexually dimorphic expression of MC3R in humans.
  • Legends for data files S1 and S2
  • Legends for movies S1 to S4
  • References (51, 52)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.pdf format). Supplementary statistical section.
  • Data file S2 (Microsoft Excel format). Raw data.
  • Movie S1 (.mov format). Three-dimensional visualization of MC3R neuronal distribution in the hypothalamus.
  • Movie S2 (.mov format). Scrolling resampling of the same three-dimensional dataset shown in movie S1 in the coronal plane.
  • Movie S3 (.mp4 format). In vivo neuronal activity of PVN MC4R neurons after a control saline injection.
  • Movie S4 (.mp4 format). In vivo neuronal activity of PVN MC4R neurons after injection of C18 (10 mg/kg, ip).