Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of rAFSC-EVs and rMSC-EVs and effects on lung explants based on size and concentration.
  • Fig. S2. Influence of rAFSCs in coculture with ex vivo and in vitro models of pulmonary hypoplasia and effects on rAFSC-EV miRNA cargo.
  • Fig. S3. Effects of rAFSC-EVs on control lung explants and primary lung epithelial cells.
  • Fig. S4. Effects of rMSC-EV administration on in vitro and ex vivo models of pulmonary hypoplasia.
  • Fig. S5. Analysis of protein and RNA cargo of rAFSC-EV and rMSC-EV.
  • Fig. S6. Enrichment plots for RNA-sequencing analysis of rMSC-EV–treated primary lung epithelial cells.
  • Fig. S7. Correlation analysis of miRNA-mRNA sequencing in primary lung epithelial cells.
  • Fig. S8. Effects of rMSC-EV administration in the in vivo model of pulmonary hypoplasia.
  • Fig. S9. Effects of hAFSC-EVs and hMSC-EVs on human fetal lung epithelial cells and in the in vivo model of pulmonary hypoplasia.
  • Table S1. Highlighted proteins expressed in rAFSC-EVs and rMSC-EVs.
  • Table S2. miRNAs related to lung development that are differentially expressed in rAFSC-EVs over rMSC-EVs.
  • Table S3. miRNAs known to be involved in pulmonary hypoplasia and present in rAFSC-EVs.
  • Table S4. Genes differentially expressed in nitrofen-injured lung epithelial cells.
  • Table S5. Interspecies conservation of top enriched miRNA in hAFSC-EVs.
  • Table S6. Primer sequences used in this study.
  • Table S7. Details of antibodies used in this study.
  • Legends for movies S1 to S6
  • Legends for data files S1 to S5
  • References (65103)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). rAFSC-EV tracking into lung explant.
  • Movie S2 (.mp4 format). Live tracking of rAFSC small EV RNA into lung epithelial cells.
  • Movie S3 (.mp4 format). Live tracking of rAFSC small EV protein into lung epithelial cells.
  • Movie S4 (.mp4 format). Live tracking of rAFSC medium/large EV RNA into lung epithelial cells.
  • Movie S5 (.mp4 format). Live tracking of rMSC small EV RNA into lung epithelial cells.
  • Movie S6 (.mp4 format). In vivo administration of AFSC-EVs before tracheal ligation in fetal rabbits.
  • Data file S1 (Microsoft Excel format). Differential analysis of rAFSC-EV and rMSC-EV protein cargo using proteomic analysis.
  • Data file S2 (Microsoft Excel format). Quality control table for all RNA-sequencing experiments used in this study.
  • Data file S3 (Microsoft Excel format). Full list of differentially expressed genes with pairwise comparisons between Nitrofen versus Nitrofen + rAFSC-EVs and Nitrofen versus Nitrofen + rMSC-EVs.
  • Data file S4 (Microsoft Excel format). Full list of pathway enrichments for mRNA-sequencing experiments.
  • Data file S5 (Microsoft Excel format). Data for Western blot experiments.