Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic depiction of EBOV D4 assay.
  • Fig. S2. D4 assay procedure.
  • Fig. S3. Mouse IgG titers for sGP and reactivity of individual scFv-phage clones for EBOV and SUDV sGP.
  • Fig. S4. scFv-Fc Ab expression, purification, and activity.
  • Fig. S5. Binding constants of scFv-Fc Abs for sGP.
  • Fig. S6. Ab pairing dose-response curves.
  • Fig. S7. Expression and purification of A1F3-1 and C2BA5-2 monoclonal IgG2a Abs.
  • Fig. S8. Binding constants of A1F3-1 (cAb) and C2BA5-2 (dAb) with multiple sGPs.
  • Fig. S9. Binding profiles of other scFv-Fc Abs with sGP.
  • Fig. S10. EBOV D4 assay performance.
  • Fig. S11. Post-assay fluorescence stability.
  • Fig. S12. EBOV D4 assay performance in human blood.
  • Fig. S13. EBOV sGP lateral flow assay fabrication.
  • Fig. S14. D4Scope design.
  • Fig. S15. Comparison of D4Scope, GenePix, and SensoSpot performance.
  • Fig. S16. RT-PCR and plaque assay.
  • Fig. S17. VP30- and GP-targeting RT-PCR.
  • Fig. S18. Western blot of MoS samples.
  • Fig. S19. Irradiated samples from EBOV-challenged nonhuman primates.
  • Table S1. D4Scope cost estimate.
  • Table S2. Summary of nonhuman primate model results.
  • Table S3. Summary of irradiated samples from EBOV-challenged nonhuman primates.
  • Legend for data file S1
  • References (7580)

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Other Supplementary Material for this manuscript includes the following: