Supplementary Materials
The PDF file includes:
- Fig. S1. Selective HDAC8 inhibition attenuates orthotopic tumor growth in immunocompetent but not immunodeficient mice.
- Fig. S2. HDAC8 inhibition has limited effect on myeloid cell proportions.
- Fig. S3. Cytolytic molecule production by tumor-infiltrating CD8+ T cells.
- Fig. S4. Effect of HDAC8 inhibition on tumor cell apoptosis in immunocompetent and immunodeficient mice.
- Fig. S5. Effect of HDAC8 inhibition on tumor cell proliferation in immunocompetent and immunodeficient mice.
- Fig. S6. Intratumoral CD8/Treg ratio determines the antitumor effect of HDAC8 inhibition.
- Fig. S7. Saturation analyses for sample number estimation.
- Fig. S8. Integrative analysis of the eight patients with NAFLD-HCC with HDAC8 overexpression.
- Fig. S9. Correlative expression analysis between HDAC8 and the 1251 enhancer-silenced genes.
- Fig. S10. Effects of HDAC8 inhibition on chemokine expressions in vitro and in vivo.
- Fig. S11. Expression of CCL4 protein in normal livers and tumors with or without HDAC8 inhibition.
- Fig. S12. Effect of CCL4 on tumor cell growth and T cell migration.
- Fig. S13. Knockdown of tumor-expressed HDAC8 has limited effects on CD8+ T cell proportions in dLN.
- Fig. S14. Effects of selective-HDAC8 and pan-HDAC inhibitors in vivo and in vitro.
- Fig. S15. Effects of HDAC8 and PD-L1 coblockade in a spontaneous NAFLD-HCC model.
- Fig. S16. A schematic diagram of this study.
- Table S1. Clinicopathological information of patients with NAFLD-HCC.
- Table S2. Primers used in this study.
- Table S3. Antibodies used in this study.
Other Supplementary Material for this manuscript includes the following:
- Data file S1 (Microsoft Excel format). List of 1251 silenced enhancer-target gene pairs in human HCCs.
- Data file S2 (Microsoft Excel format). Original data.