Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. mtDNA distribution in human peripheral blood cells.
  • Fig. S2. NETs in patients with SLE with higher disease activity and in healthy volunteers.
  • Fig. S3. Benzonase digests isolated mtDNA.
  • Fig. S4. Flow cytometry analysis and the gating strategy used for the detection of circulating extracellular vesicles.
  • Fig. S5. Lupus pathogenesis is B/WF1 mice expressing FcγRIIA.
  • Fig. S6. Circulating AwMA and AmtDNA antibodies.
  • Fig. S7. Representative images of immunofluorescence in kidneys.
  • Fig. S8. Plasma concentrations of mtDNA increase in an FcγRIIA-dependent mechanism before signs of nephritis in mice with SLE.
  • Fig. S9. LL37 quantification in vitro and in vivo.
  • Fig. S10. Mitochondria accumulate in the kidneys and lungs in mice with SLE.
  • Fig. S11. Quantification of mtDNA and antibodies targeting mitochondrial antigens in urine.
  • Fig. S12. Murine platelets expressing FcγRIIA release mitochondria in vitro.
  • Fig. S13. DsRed+ EV released in vivo do not express CD45 and their release is not enhanced by TLR9 stimulation.
  • Table S1. Demographic variables of patients included in the study.
  • Table S2. Clinical features of patients included in the study.
  • Legend for data file S1
  • References (6164)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Raw data (provided as a separate Excel file).