Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. mtDNA distribution in human peripheral blood cells.
- Fig. S2. NETs in patients with SLE with higher disease activity and in healthy volunteers.
- Fig. S3. Benzonase digests isolated mtDNA.
- Fig. S4. Flow cytometry analysis and the gating strategy used for the detection of circulating extracellular vesicles.
- Fig. S5. Lupus pathogenesis is B/WF1 mice expressing FcγRIIA.
- Fig. S6. Circulating AwMA and AmtDNA antibodies.
- Fig. S7. Representative images of immunofluorescence in kidneys.
- Fig. S8. Plasma concentrations of mtDNA increase in an FcγRIIA-dependent mechanism before signs of nephritis in mice with SLE.
- Fig. S9. LL37 quantification in vitro and in vivo.
- Fig. S10. Mitochondria accumulate in the kidneys and lungs in mice with SLE.
- Fig. S11. Quantification of mtDNA and antibodies targeting mitochondrial antigens in urine.
- Fig. S12. Murine platelets expressing FcγRIIA release mitochondria in vitro.
- Fig. S13. DsRed+ EV released in vivo do not express CD45 and their release is not enhanced by TLR9 stimulation.
- Table S1. Demographic variables of patients included in the study.
- Table S2. Clinical features of patients included in the study.
- Legend for data file S1
- References (61–64)
Other Supplementary Material for this manuscript includes the following:
- Data file S1. Raw data (provided as a separate Excel file).
