Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. CXCR3 abundance and tumor-related reactivity of memory CD8+ T cell subpopulations in lymph nodes of patients with BC.
  • Fig. S2. Enrichment of the CXCR3 ligands in lymph nodes of patients with MIBC associates with tumor drainage.
  • Fig. S3. CXCL11 induces migration, activation, and proliferation in early differentiated CD8+ T cells.
  • Fig. S4. CXCL11 induces down-regulation of CXCR3 in early differentiated CD8+ T cells.
  • Fig. S5. CXCL4 does not alter CD8+ TSCM function.
  • Fig. S6. CXCL11-specific receptors DARC and CXCR7, the RT-qPCR panel designed to analyze the CXCR3 isoforms, and CXCR3 isoform expression in CD8+ T cells of patients with BC.
  • Fig. S7. Patient work-up and performance criteria of patients with BC.
  • Fig. S8. Intratumoral cytokine milieus differ between BC patient groups.
  • Fig. S9. Pretreatment serum cytokine abundance of patients with BC.
  • Fig. S10. Intratumoral CXCL11high state predicts improved OS in a second independent cohort of patients with MIBC.
  • Fig. S11. Intratumoral CXCL4 state does not associate with OS in a second independent cohort of patients with MIBC.
  • Fig. S12. CXCR3 expression associates with tissue-infiltrating CD8+ T cells in healthy bladder and in tumor-infiltrating CD8+ T cells in MIBC.
  • Fig. S13. CXCR3 expression associates with tumor-infiltrating CD8+ T cells and CXCL9, 10, and 11 expression by macrophages in melanoma.
  • Fig. S14. Intratumoral CXCR3 expression in relation to CD8+ T cell differentiation state in solid tumors.
  • Fig. S15. Enrichment of the CXCR3 isoforms and CD3 in lymph nodes of patients with MIBC associates with tumor drainage.
  • Fig. S16. Graphical summary.
  • References (80–95)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual data for experiments with n < 20.
  • Data file S2 (Microsoft Excel format). Patient characteristics.
  • Data file S3 (Microsoft Excel format). Analytes applied for protein measurement in the Luminex system.
  • Data file S4 (Microsoft Excel format). Intratumoral cytokines in relation to NAC response and T cell infiltration.

More From Science Translational Medicine