Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Efficient HAdv-C5 virus sequestration in liver macrophages requires natural IgM antibodies and scavenger receptor CD36.
  • Fig. S2. Hexon HVR1 sequence comparison for WT and mutated adenoviruses.
  • Fig. S3. Schematic diagrams of the genomic structure of viral vectors and specific mutations introduced into the hexon and penton base capsid proteins of each adenovirus variant.
  • Fig. S4. Penton base loop sequence comparison for WT and mutated adenoviruses.
  • Fig. S5. Structural modeling and MD simulations of the penton base containing the Lam1 insertion.
  • Fig. S6. Modeling of penton base loop in HAdv-C5.
  • Fig. S7. HAdv-C5 retargeting from β3 integrins to integrin interactions mediated by SIKVAV reduces inflammation.
  • Fig. S8. Resolution assessment of the Ad5-3M capsid.
  • Fig. S9. Local resolution assessment of Ad5-3M.
  • Fig. S10. Comparison of Ad5-3M hexon and penton base with WT protein structures.
  • Fig. S11. Human sera with high HAdv-C5–specific neutralizing antibody titers neutralize Ad5-WT and capsid-modified adenoviruses.
  • Fig. S12. Ad5-3M effectively infects various NSCLC cell lines.
  • Fig. S13. Ad5-3M escapes Kupffer cell sequestration.
  • Fig. S14. Ad5-3M biodistribution after intravenous delivery.
  • Fig. S15. A549-Luc-C8 cells form tumor nodules in lungs after intravenous injection.
  • Fig. S16. Intravenous administration of replication competent Ad5/35-3M virus does not trigger systemic toxicity.
  • Table S1. Mouse strains used in the study.
  • Table S2. List of antibodies used in the study.
  • Table S3. Sequences of the qPCR primers.
  • Legends for data files S1 to S3
  • References (5766)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Statistical summary report. (Microsoft Excel format).
  • Data file S2. Primary data for main figures of the paper. (Microsoft Excel format).
  • Data file S3. Primary data for figures shown in Supplementary Materials. (Microsoft Excel format).