Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. The concentration of RhebCA influences h current amplitudes.
  • Fig. S2. HCN1 to HCN3 immunostaining.
  • Fig. S3. siRNA validation of HCN4 antibodies.
  • Fig. S4. HCN4 antibodies detect HCN4 overexpression.
  • Fig. S5. HCN4 immunostaining in patient 2 with FCDII.
  • Fig. S6. HCN4 immunostaining in patients 3 to 5 with FCDII.
  • Fig. S7. HCN4 and phospho-S6 or SMI-311 immunostaining in patient 7 with FCDII.
  • Fig. S8. HCN4 overexpression does not lead to convulsive seizures.
  • Table S1. Patient information.
  • Table S2. Constructs used.
  • Table S3. Primary and secondary antibodies.
  • Table S4. Summary of statistical tests.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Example of a convulsive seizure in a mouse containing an FCM.
  • Data file S1 (Microsoft Excel format). Raw data.