Supplementary Materials

The PDF file includes:

  • Fig. S1. Genome sequence and fitness analysis of PBT2 + polymyxin–resistant P. aeruginosa MS14403 mutants.
  • Fig. S2. RNA transcriptome analysis of K. pneumoniae strain MS6671 in response to treatment with PBT2 and colistin.
  • Fig. S3. Effects of PBT2 on outer-membrane zeta potential, bacterial size, and outer-membrane permeability.
  • Fig. S4. K. pneumoniae strains MS6671 and 52.145∆mgrB lipid A MALDI-TOF MS analysis.
  • Fig. S5. K. pneumoniae strain MS6671 colonizes BALB/c murine skin but is avirulent in the CD1 mouse systemic infection model.
  • Table S1. PBT2 resensitizes pandrug-resistant K. pneumoniae strain MS6671 to polymyxin class antibiotics in the absence of zinc.
  • Table S2. FICI values for PBT2 + polymyxin combinations.
  • Table S3. PBT2 polymyxin potentiator activity against polymyxin-resistant Gram-negative pathogens in the absence and presence of zinc, iron, manganese, or copper.
  • Table S4. MIC of polymyxin class antibiotics in the presence and absence of zinc, against polymyxin-resistant Gram-negative pathogens.
  • Table S5. K. pneumoniae MS6671 up-regulated genes after 1-hour treatment with 64 μM PBT2 in CA-MHB grown at 37°C.
  • Table S6. K. pneumoniae MS6671 down-regulated genes after 1-hour treatment with 64 μM PBT2 in CA-MHB grown at 37°C.
  • Table S7. K. pneumoniae MS6671 down-regulated genes after 1-hour treatment with colistin (0.25 μg/ml) in CA-MHB grown at 37°C.
  • Table S8. K. pneumoniae MS6671 up-regulated genes after 1-hour treatment with PBT2 (64 μM) and colistin (0.25 μg/ml) in CA-MHB grown at 37°C.
  • Table S9. K. pneumoniae MS6671 down-regulated genes after 1-hour treatment with PBT2 (64 μM) and colistin (0.25 μg/ml) in CA-MHB grown at 37°C.
  • Table S10. The relative percentage abundance of K. pneumoniae MS6671 and K. pneumoniae 52.145∆mgrB lipid A ions observed using MALDI-TOF mass spectrometry.
  • Table S11. PBT2 resensitizes polymyxin-resistant K. pneumoniae strain 52.145∆mgrB to polymyxins.
  • Table S12. Real-time PCR primers used in this study.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Raw data for all figures.