Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. Representative kymographs for BACE1-mCherry and GFP-GGA3 cotransport experiments in Fig. 2D.
- Fig. S2. Representative images for PI analysis in Fig. 3B.
- Fig. S3. Colocalization analysis for BACE1-mCherry with EEA1 and LAMP2.
- Fig. S4. Representative image for BACE1-mCherry and LAMP2 in Gga3−/− axonal swelling.
- Fig. S5. NFH staining of hippocampal CA1 axonal bundles and BACE1 accumulation in the cerebellum and hippocampus of Gga3−/− mice.
- Fig. S6. Images for BACE1-mcherry and GFP-GGA3 channels corresponding to axon in Fig. 6A.
- Fig. S7. Supplementary data and analysis related to BACE inhibition experiments.
- Fig. S8. Representative images of cerebella and hippocampi from MBi-3–treated mice.
- Fig. S9. Images for BACE1-mcherry and GFP-GGA3 channels corresponding to axons in Fig. 7C.
- Table S1. [MBi-3] micromolars—Pharmacokinetics (PK) analysis summary.
- Table S2. Genetics dataset description.
- Table S3. Selected single variants, showing nominal association with AD in one of the datasets.
- Table S4. Summary of the antibodies used throughout this study.
- Legend for data file S1
- References (60–64)
Other Supplementary Material for this manuscript includes the following:
- Movie S1 (.mp4 format). BACE1-mCherry and GFP-GGA3 axonal trafficking in wild-type neurons.
- Movie S2 (.mp4 format). BACE1-mCherry and synaptophysin-GFP axonal trafficking in Gga3+/+ and Gga3−/− neurons.
- Movie S3 (.mov format). Three-dimensional reconstruction of BACE1-mCherry and LAMP2 in Gga3−/− axonal swelling.
- Movie S4 (.mp4 format). BACE1-mCherry and synaptophysin-GFP accumulation in axonal swellings in Gga3−/− neurons.
- Movie S5 (.mp4 format). Representative movies for BACE1-mCherry trafficking experiments in Fig. 6.
- Movie S6 (.mp4 format). Representative movies for BACE1-mCherry trafficking experiments in Fig. 7.
- Data file S1. Individual-level data file n < 20 (provided as separate Excel file).