Supplementary Materials

This PDF file includes:

  • Fig. S1. Subsets of ACPA-positive B cells in synovial fluid of inflamed joints from patients with RA.
  • Fig. S2. Histogram plots depicting the positivity for CD80, CD86, or Ki-67 in ACPA-positive (ACPA+), TT-specific (TT+), and bulk (ACPA) MBC populations.
  • Fig. S3. Relative gene expression of B cell phenotypic markers in ACPA-positive and non–citrulline-reactive MBCs.
  • Fig. S4. Correlation analysis between systemic inflammation and the characteristics of ACPA-positive MBCs.
  • Fig. S5. Characteristics of ACPA-positive B cells in individuals with ACPA+ arthralgia.
  • Fig. S6. Phenotype of ACPA-positive B cells in different disease phases compared to TT-specific B cells in the steady state and upon recent vaccination in patients with RA.
  • Fig. S7. Correlation between the proportion of TT-specific MBCs positive for CD80 or CD86, and abundance of CD19 or HLA-DR and time after TT vaccination in untreated patients with RA.
  • Fig. S8. Cytokine secretion by ACPA-positive MBCs.
  • Fig. S9. Abundance of IgG on CD20+CD27+ ACPA-positive and ACPA-negative B cells from RA patient peripheral blood.
  • Fig. S10. Production of IL-8 by TT-specific and ACPA-positive, immortalized B cell clones triggered by various stimulants.
  • Fig. S11. Production of IL-8 by B cells from healthy individuals under different stimulation conditions and at different time points after stimulation.
  • Data file S1. Subject-level data for experiments with n < 20 donors or replicates.

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