Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Competition assay between C. acnes and S. aureus.
  • Fig. S2. HPLC chromatogram of cutimycin purification.
  • Fig. S3. Low-energy MS of cutimycin.
  • Fig. S4. 1H NMR spectrum of cutimycin.
  • Fig. S5. 13C NMR spectrum of cutimycin.
  • Fig. S6. 1H-1H COSY NMR of cutimycin.
  • Fig. S7. 1H-13C HSQC NMR of cutimycin.
  • Fig. S8. 1H-13C HMBC NMR of cutimycin.
  • Fig. S9. Identification of dehydroalanine residues.
  • Fig. S10. Weak four-bond HMBC correlations.
  • Fig. S11. Marfey’s analysis of cutimycin.
  • Fig. S12. Structure of cutimycin.
  • Fig. S13. Structures of cutimycin, berninamycin A, and LFF-571.
  • Fig. S14. Calibration curve for the +1 ion of the cutimycin standard.
  • Fig. S15. Metagenomics analysis of C. acnes BGCs on human skin.
  • Fig. S16. Effect of cutimycin BGC presence on C. acnes, C. granulosum, and S. epidermidis CFUs in human skin hair follicles.
  • Fig. S17. Linear depiction of cutimycin.
  • Table S1. The presence or absence of the cutimycin BGC in 219 Cutibacterium genomes.
  • Table S2. NMR data of cutimycin in DMSO-d6 at 600 and 151 MHz.
  • Table S3. Detection of cutimycin in samples of human skin hair follicles.
  • Table S4. Locus tags for the C. acnes BGCs in human skin metagenomics data.
  • Table S5. Presence or absence of the cutimycin BGC and C. acnes, C. granulosum, and S. epidermidis CFUs in human skin hair follicles.
  • Table S6. Bacterial strains and plasmids used in this study.
  • Table S7. Primers used in this study.
  • Table S8. Key MS fragments confirming the amino acid order in cutimycin.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual-level data for Fig. 2.