Supplementary Materials

The PDF file includes:

  • Fig. S1. Longitudinal analysis of ZIKV kinetics in the blood of dams.
  • Fig. S2. ZIKV tissue tropism in dam-fetal pairs at delivery.
  • Fig. S3. Longitudinal analysis of fetal biparietal brain diameter.
  • Fig. S4. MRI of macaque brains.
  • Fig. S5. Validation of macaque antibody specificity in a SPR assay.
  • Fig. S6. Dam and infant ZIKV envelope and NS1 protein–specific antibody isotype measurements.
  • Fig. S7. Flow cytometry gating strategy for ZIKV-specific memory T cells.
  • Fig. S8. Spinal cord lesions in macaque offspring reinfected with ZIKV.
  • Fig. S9. Neutralization and antibody-dependent enhancement in infant blood before and after ZIKV reinfection.
  • Fig. S10. Mineralization of brain basal ganglia in macaque offspring reinfected with ZIKV.
  • Table S1. Scoring of tissue pathology for macaque dams at the time of delivery.
  • Table S2. Scoring of tissue pathology for macaque offspring at the time of delivery.
  • Table S3. qPCR results for detection of ZIKV RNA in CSF of macaque offspring.
  • Table S4. Results of ophthalmic exams.
  • Table S5. Scoring of CNS tissue pathology for macaque offspring reinfected with ZIKV.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Brazelton exam assessment for infant macaque behavior tests.
  • Data file S2 (Microsoft Excel format). Scoring of non-CNS tissue pathology for macaques after ZIKV reinfection.