Supplementary Materials

The PDF file includes:

  • Fig. S1. ME TFs compared with GTEx tissues and articular cartilage.
  • Fig. S2. Evaluation of MKX expression between human cartilage zones.
  • Fig. S3. Viability assay and gene expression in human BM-MSCs transduced with Ad-MKX and microscopy of Ad-GFP–transduced BM-MSCs.
  • Fig. S4. mRNA expression in Ad-MKX–transduced human SYN-MSCs after 10-day monolayer culture.
  • Fig. S5. Comparative analysis of RNA-seq count values between human meniscus and cartilage.
  • Fig. S6. qRT-PCR analysis of Ad-MKX–transduced human vascular and avascular cells.
  • Fig. S7. Decellularization, heparin, and TGF-β3 conjugation of bovine meniscus tissue.
  • Fig. S8. Cellularity of MSC-seeded DMS.
  • Fig. S9. Histology and GFP fluorescence of mouse knee joints.
  • Fig. S10. Safranin-O staining and immunohistochemistry of Ad-GFP– or Ad-MKX–injected mouse knee joints after DMM surgery.
  • Fig. S11. Safranin-O staining of Ad-GFP– or Ad-MKX–injected mouse knee joints after DMM surgery.
  • Fig. S12. Immunohistochemistry of Ad-GFP– or Ad-MKX–injected mouse knee joints after DMM surgery.
  • Fig. S13. Immunohistochemistry and histology of Ad-GFP– or Ad-MKX–injected human meniscus explants from patients with OA.
  • Fig. S14. Density plot of log2CPM expression values for all tissues.
  • Fig. S15. Gene expression in tissues above thresholds.
  • Fig. S16. Negative controls for immunohistochemistry.
  • Fig. S17. Immunohistochemistry of DMS in bovine meniscus explant.
  • Table S1. ME TFs compared with GTEx tissues.
  • Table S2. ME TFs compared with GTEx tissue and articular cartilage.
  • Table S3. TaqMan qRT-PCR probe information.

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Other Supplementary Material for this manuscript includes the following: