Supplementary Materials

The PDF file includes:

  • Materials and methods
  • Fig. S1. FcRn is expressed in human epithelial cells.
  • Fig. S2. T84 cells do not express surface CD32 or CD64.
  • Fig. S3. A schematic illustration of the transcytosis assay.
  • Fig. S4. Intranasal delivery of droplets reaches the lungs of WT mice.
  • Fig. S5. The co-crystal structure of binding of WT and mutant albumin to FcRn.
  • Fig. S6. Circular dichroism spectra of albumin variants.
  • Fig. S7. Human FcRn binding and transport properties of albumin-GST fusions.
  • Fig. S8. Pulmonary delivery of albumin and IgG1 to Tg32 alb KO mice.
  • Fig. S9. Non-reduced SDS-PAGE of different albumin formats.
  • Fig. S10. Binding of scFv-albumin fusions to human FcRn and pulmonary delivery of an Fab fragment to Tg32 alb KO mice.
  • Fig. S11. QMP is rescued from intracellular degradation in an FcRn-dependent manner.
  • Fig. S12. Schematic representation of physiological versus defective thrombin generation restored by rFVIIa-albumin in hemophilia.
  • Fig. S13. SDS-PAGE analysis of rFVIIa-albumin fusions.
  • Fig. S14. Binding of rFVIIa-albumin fusions to human and mouse FcRn.
  • Fig. S15. rFVIIa-QMP shows extended half-life in BALB/c mice.
  • Fig. S16. Binding of rFVIIa-albumin fusions to monkey FcRn.
  • Table S1. The content of α-helix and random coil determined by CD at pH 5.5.
  • References (6478)

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