Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. LZCM and α-synuclein fibril do not induce LRRK2 phosphorylation in microglia.
  • Fig. S2. TLR2-dependent LRRK2 phosphorylation by neuron-released α-synuclein in microglia.
  • Fig. S3. LRRK2 modulates microglial uptake of extracellular α-synuclein.
  • Fig. S4. Quantitative analysis of microglia cytokine gene expression in primary microglia exposed to LRRK2 kinase inhibitor.
  • Fig. S5. Generation and analysis of microglia conditioned medium.
  • Fig. S6. TLR2-dependent induction and nuclear translocation of NFATc2 in α-synuclein–exposed microglia.
  • Fig. S7. Calcineurin-independent induction of NFATc2 in α-synuclein–exposed microglia.
  • Fig. S8. NFATc2 induction in α-synuclein exposed isolated aged microglia.
  • Fig. S9. An example of MS/MS data obtained for phosphopeptides detected in NFATc2.
  • Fig. S10. Schematic illustration of LRRK2 kinase inhibitor administration.
  • Fig. S11. LRRK2 kinase inhibitor administration reduces nuclear localization of NFATc2 in the brain of a synucleinopathy mouse model.
  • Fig. S12. Model for microglia-mediated neurotoxicity in synucleinopathies.
  • Table S1. Human specimens, neuropathological evaluation, and criteria for diagnosis.
  • Table S2. List of primers for quantitative polymerase chain reaction.
  • Legend for data file S1
  • References (7290)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Raw data (provided as supplementary Excel file).