Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Differentially expressed EEV protein content in normoxic and hypoxic conditions.
  • Fig. S2. Molecular function GO classification of DEPs in hCMs treated with EEVs compared to untreated controls.
  • Fig. S3. Biological process GO classification of DEPs in hCMs treated with EEVs compared to untreated controls.
  • Fig. S4. ROS measurement in menadione-challenged human cardiomyocytes.
  • Fig. S5. Preconditioning versus EEV treatment effect on viability after IRI.
  • Fig. S6. Molecular function GO classification of DEPs in injured hCMs pretreated with EEVs compared with injured, untreated hCMs.
  • Fig. S7. Biological process GO classification of DEPs in injured hCMs pretreated with EEVs compared with injured, untreated hCMs.
  • Fig. S8. EEV uptake by human cardiomyocytes.
  • Fig. S9. Heart-on-a-chip device for contractility measurements.
  • Fig. S10. Nonsignificant cardioprotective effect of human EEVs on neonate rat ventricular myocyte–based heart-on-a-chip assay.
  • Table S1. Common contaminant proteins detected in EEV samples.
  • Table S2. GO terms and enrichment P values for overrepresented protein groups presented in Fig. 2 (C and D) with corresponding color coding.
  • Table S3. Cardioprotective function of protein groups represented in EEVs.
  • Table S4. Possible cardioprotective miRNA cargo in previously published studies.
  • Legends for movies S1 to S3
  • Legends for data files S1 to S4
  • References (7587)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). EEV uptake by human cardiomyocytes (cell 1).
  • Movie S2 (.avi format). EEV uptake by human cardiomyocytes (cell 2).
  • Movie S3 (.avi format). EEV uptake by human cardiomyocytes (cell 3).
  • Data file S1 (Microsoft Excel format). Total EEV proteome raw data.
  • Data file S2 (Microsoft Excel format). DEPs in EEV-treated versus untreated hCMs.
  • Data file S3 (Microsoft Excel format). DEPs in IR-injured, EEV-treated versus injured, untreated hCMs.
  • Data file S4 (Microsoft Excel format). Summary of statistical analyses and P values.