Supplementary Materials

The PDF file includes:

  • Fig. S1. Effective coating of MPIO with anti–MAdCAM-1 monoclonal antibodies as assessed by flow cytometry.
  • Fig. S2. Effective coating of MPIO with anti–MAdCAM-1 monoclonal antibodies as assessed by confocal microscopy.
  • Fig. S3. Intraperitoneal LPS administration induces MAdCAM-1 overexpression in the ileum.
  • Fig. S4. MPIO-αMAdCAM-1 bind to MAdCAM-1–expressing vessels after intravenous injection in the LPS model.
  • Fig. S5. Multislice T2*-weighted images before and after MPIO-αMAdCAM-1 administration in an LPS-treated mouse.
  • Fig. S6. Longitudinal imaging after a single injection of MPIO-αMAdCAM-1 in LPS-treated mice.
  • Fig. S7. Multislice T2- and T2*-weighted images before and after MPIO-αMAdCAM-1 administration in the DSS model of acute colitis.
  • Fig. S8. MPIO-αMAdCAM-1 bind specifically to the inflammatory mucosa in the DSS model of acute colitis.
  • Fig. S9. Sonication of MPIO-αMAdCAM-1 prevents the trapping of aggregates in the microcirculation in the DSS model of acute colitis.
  • Fig. S10. Analysis of the binding kinetic of MPIO-αMAdCAM-1 in the DSS model of acute colitis.
  • Fig. S11. Influence of antibody concentration on the surface of MPIO-αMAdCAM-1 on binding and clearance from the endothelial surface in the DSS model of acute colitis.
  • Fig. S12. Blockade of integrin α4β7 and MPIO-αMAdCAM-1 binding in the DSS-induced model of acute colitis.
  • Fig. S13. Schematic representation of the main findings.
  • Legends for movies S1 and S2

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). MPIO-αMAdCAM-1 binds to the activated endothelium of the gastrointestinal tract.
  • Movie S2 (.mp4 format). Binding kinetic of MPIO-αMAdCAM-1 in the DSS model of acute colitis.
  • Data file S1 (Microsoft Excel format). Individual-level data.