Supplementary Materials

The PDF file includes:

• Materials and methods
• Fig. S1. ER cells that have undergone EMT display EGFR independence but have not undergone SCLC transformation.
• Fig. S2. YAP but not YAP regulators are up-regulated in EGFR TKI–resistant cells that have undergone EMT.
• Fig. S3. YAP activity is increased in EGFR TKI–resistant cells that have undergone EMT and in drug-tolerant persister cells.
• Fig. S4. HCC827 ER and HCC4006 ER cells acquired a multidrug-resistant phenotype.
• Fig. S5. HCC4006 ER cells are sensitive to SAC component inhibitors.
• Fig. S6. HCC827 ER cells are sensitive to SAC component inhibitors.
• Fig. S7. Cell line models and patient-derived models of acquired osimertinib resistance are sensitive to inhibition of SAC components.
• Fig. S8. CDK and SAC component inhibitors induced tumor cell death and increased nuclear size.
• Fig. S9. FOXM1 regulates SAC component gene expression and promotes osimertinib resistance.
• Fig. S10. FOXM1 expression correlates with expression of genes encoding SAC components in clinical specimens and is associated with worse overall survival in patients with EGFR-mutant NSCLC.
• Table S1. Multivariate analysis of FOXM1, DFS, and stage.
• Legends for data files S1 to S11
• References (64–73)

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Acquired mutations in ER cells.
• Data file S2 (Microsoft Excel format). IC₇₀ values of drugs with acquired resistance in HCC4006 ER cells.
• Data file S3 (Microsoft Excel format). IC₇₀ values of drugs with acquired resistance in HCC827 ER cells.
• Data file S4 (Microsoft Excel format). IC₅₀ and IC₇₀ values of drugs for PC9 and PC9 ER cells.
• Data file S5 (Microsoft Excel format). IC₅₀ and IC₇₀ values of drugs for HCC827 control and HCC827 ZEB1 cells.
• Data file S6 (Microsoft Excel format). Fold change in IC₇₀ values for HCC4006 ER versus HCC4006 cells.
• Data file S7 (Microsoft Excel format). Fold change in IC₇₀ values for HCC827 ER versus HCC827 cells.
• Data file S8 (Microsoft Excel format). GI₅₀ values of drugs with sustained sensitivities in HCC827 ER cells.
• Data file S9 (Microsoft Excel format). GI₅₀ values of drugs with sustained sensitivities in HCC4006 ER cells.
• Data file S10 (Microsoft Excel format). Mean GI₅₀ and IC₇₀ values of drugs for EGFR wild-type and EGFR mutant NSCLC cell lines.
• Data file S11 (Microsoft Excel format). IC₅₀ and IC₇₀ values of drugs for HCC827 control and HCC827 FOXM1 cells.