Supplementary Materials

The PDF file includes:

  • Fig. S1. A control ASO showed dose-responsive effect on SMN2 transcript splicing in ReNcells.
  • Fig. S2. Dose-dependent effects of ASO-22 on expression of Scn1a gene in ICV-injected neonatal mouse brains.
  • Fig. S3. Dose-dependent effects of ASO-22 on expression of NaV1.1 in ICV-injected neonatal mouse brains.
  • Fig. S4. A control ASO promoted exon 35 exclusion of Cep290 mRNA in neonatal mouse brain.
  • Fig. S5. Expression of Scn1a mRNA in mouse brains at different post-injection days.
  • Fig. S6. Expression of NaV1.1 in mouse brains at different post-injection days.
  • Fig. S7. Numbers of Scn1a+/− mice that had 0, 1, or 2+ seizures after treatment.
  • Fig. S8. Validation of the two anti-NaV1.1 antibodies.
  • References (29, 30)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Terminal seizure event in a DS mouse injected with 60 μg of ASO at P14.
  • Movie S2 (.avi format). Terminal seizure event in a DS mouse injected with 3 μl of PBS at P14.
  • Data file S1 (Microsoft Excel format). Individual-level data for figures with n less than 20.