Supplementary Materials

The PDF file includes:

  • Fig. S1. Surface expression of signaling domain CAR variants with different transmembrane domains or scFvs.
  • Fig. S2. Supporting information for xenoGVHD model.
  • Fig. S3. hCD45 engraftment in xenoGVHD model.
  • Fig. S4. HLA-A2+ cell engraftment in xenoGVHD model.
  • Fig. S5. Myc+hCD45+ cells in GVHD model.
  • Fig. S6. Activation of human Tregs by signaling domain CAR variants.
  • Fig. S7. Cytokine profile of signaling domain CAR variant–stimulated human Tregs.
  • Fig. S8. Effect of CAR-mediated expansion on markers of memory and exhaustion.
  • Fig. S9. Supporting information for suppression assays.
  • Fig. S10. Effects of signaling domain CAR variants on the Treg transcriptome.
  • Fig. S11. Transcriptome analysis of CAR- and TCR-stimulated Tregs and Tconv.
  • Table S1. Amino acid sequences for cassettes used in generation of co-receptor CAR constructs.
  • Table S2. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting Fig. 7A.
  • Table S3. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting Fig. 7B.
  • Table S4. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting Fig. 7C.
  • Table S5. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting Fig. 7D.
  • Table S6. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting fig. S10B.
  • Table S7. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting fig. S10C.
  • Table S8. Gene set enrichment analysis of transcription factor targets and hallmark pathways supporting fig. S10D.
  • Table S9. Antibodies used in flow cytometry analyses for in vitro studies.
  • Table S10. Antibodies used in flow cytometry analyses for in vivo studies.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Primary data from main figures.
  • Data file S2 (Microsoft Excel format). Primary data from supplementary figures.