Supplementary Materials

The PDF file includes:

• Materials and Methods
• Fig. S1. Ceramide regulates the Hippo signaling pathway in human HSCs.
• Fig. S2. Ceramide and B13 promote YAP/TAZ degradation.
• Fig. S3. Ceramide promotes β-TrCP–mediated proteasomal degradation of YAP/TAZ.
• Fig. S4. aCDase deficiency in HSCs reduces CCl₄-induced fibrosis.
• Fig. S5. aCDase deficiency in HSCs reduces NASH fibrosis.
• Fig. S6. Sphingolipid analysis of HSCs after treatment with ceramide or B13.
• Fig. S7. B13 treatment reduces CCl₄-induced fibrosis.
• Fig. S8. Nortriptyline reduces CCl₄-induced fibrosis.
• Fig. S9. aCDase inhibition promotes fibrosis regression.
• Table S1. Ingenuity canonical pathways down-regulated by ceramide.

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Genes in the HSC activation pathway, Hippo/YAP pathway, and actin cytoskeleton pathway.
• Data file S2 (Microsoft Excel format). Genes in the CRS, YAP score, and HSC activation score.
• Data file S3 (Microsoft Excel format). Individual subject-level data for main figures.
• Data file S4 (Microsoft Excel format). Individual subject-level data for supplementary figures.