Supplementary Materials

The PDF file includes:

  • Fig. S1. Additional optical and scanning electron microscopy images of microparticles.
  • Fig. S2. Cumulative in vitro release kinetics of AF647-dextran from PLGA-MPs.
  • Fig. S3. Fluorescence of AF647-dextran was quenched before release from microparticles.
  • Fig. S4. Representative IVIS images of mice that received AF647-dextran–loaded PLGA-MPs.
  • Fig. S5. Averaged release time of AF647-dextran from subcutaneously injected PLGA-MPs.
  • Fig. S6. Free AF647 was rapidly cleared from tumors.
  • Fig. S7. HPLC analysis of cGAMP before and after release.
  • Fig. S8. Cumulative in vitro release of 3′3′-cGAMP from hydrogels.
  • Fig. S9. Tumor growth and survival analysis of subcutaneous 4T1 breast tumor–bearing mice treated with cGAMP-MPs.
  • Fig. S10. Systemic IL-6 concentrations in orthotopic 4T1 tumor–bearing mice after treatments.
  • Fig. S11. Representative image of B16F10 tumors and average tumor growth curve after treatments.
  • Fig. S12. Gating scheme for flow cytometry measurements of myeloid and lymphoid cells in TME.
  • Fig. S13. Quantification of lymphocytes in the TME by flow cytometry.
  • Fig. S14. Immunofluorescence images of histological sections of B16F10 tumors after treatments.
  • Fig. S15. TUNEL staining of histological sections of B16F10 tumors after treatments.
  • Fig. S16. Analysis of regulatory T cells in the TME after treatments.
  • Fig. S17. Flow cytometry measurements of T cells in systemic circulation.
  • Fig. S18. Body weight analysis of mice bearing B16F10 melanoma and 4T1 breast tumors after treatments.
  • Fig. S19. H&E-stained sections of major organs from B16F10 tumor–bearing mice after different treatments.
  • Fig. S20. H&E staining images showing PLGA microparticles deformed and completely degraded over time in vivo.
  • Fig. S21. Cumulative in vitro release kinetics of pemetrexed- and Cy5-labeled CpG DNA from PLGA-2.
  • Fig. S22. Representative scanning electron microscopy image of a thinner-walled microparticle and cGAMP loading per particle.
  • Table S1. PLGA compositions and in vitro release time points of AF647-dextran from different PLGA-MPs.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual subject-level data.